What is it? PCR or polymerase chain reaction is a technique used in molecular biology and diagnostics to amplify a single or few copies of a sequence of DNA or RNA into thousands to millions. Developed in 1983 by Kary Mullis, who also went on to win the Nobel Prize in 1993. It changed the … Continue reading PCR, A Surge Forward
An urethral swab was obtained from a man with an urethral exudate was plated directly on chocolate agar and modified Thayer-Martin agar and a gram stain was made. The culture plates were incubated at 35 degrees celsius, but showed no growth at 48 hours. The gram stain showed gram-negative diplococci. What is a Thayer-Martin agar? … Continue reading Gram-negative diplococci failed to grow at 48 hours in 35 degree Celsius.
Cytochemical stains are great tools to distinguish the different myelogenous leukemias from each other. There are multiple different stains employed to help this process. Myeloperoxidase (MPO): MPO is a lysosomal protein that is stored in the azurophilic granules of neutrophils and is released upon degranulation into the extracellular space. During respiratory burst it produces hypochlorous acid … Continue reading FAB Classification of Leukemias and Cytochemical Stain Observations in each.
A three year old child was evaluated for abdominal pain and anorexia by a physician. CBC reveals Hgb of 9.8 g/dL and basophilic stippling of the RBCs. The physician should order further tests to check for poisoning from? a) Arsenic b) Iron c) Mercury d) Lead Patient presents slightly anemic with a hemoglobin of 9.8 … Continue reading 3 year old evaluated with abdominal pain and anorexia.