PCR, A Surge Forward

What is it? PCR or polymerase chain reaction is a technique used in molecular biology and diagnostics to amplify a single or few copies of a sequence of DNA or RNA into thousands to millions. Developed in 1983 by Kary Mullis, who also went on to win the Nobel Prize in 1993. It changed the … Continue reading PCR, A Surge Forward

Gram-negative diplococci failed to grow at 48 hours in 35 degree Celsius.

An urethral swab was obtained from a man with an urethral exudate was plated directly on chocolate agar and modified Thayer-Martin agar and a gram stain was made. The culture plates were incubated at 35 degrees celsius, but showed no growth at 48 hours. The gram stain showed gram-negative diplococci. What is a Thayer-Martin agar? … Continue reading Gram-negative diplococci failed to grow at 48 hours in 35 degree Celsius.

FAB Classification of Leukemias and Cytochemical Stain Observations in each.

Cytochemical stains are great tools to distinguish the different myelogenous leukemias from each other. There are multiple different stains employed to help this process. Myeloperoxidase (MPO): MPO is a lysosomal protein that is stored in the azurophilic granules of neutrophils and is released upon degranulation into the extracellular space. During respiratory burst it produces hypochlorous acidContinue reading FAB Classification of Leukemias and Cytochemical Stain Observations in each.

3 year old evaluated with abdominal pain and anorexia.

A three year old child was evaluated for abdominal pain and anorexia by a physician. CBC reveals Hgb of 9.8 g/dL and basophilic stippling of the RBCs. The physician should order further tests to check for poisoning from? a) Arsenic b) Iron c) Mercury d) Lead Patient presents slightly anemic with a hemoglobin of 9.8 … Continue reading 3 year old evaluated with abdominal pain and anorexia.

What Is Medical Laboratory Science?

According to the American Society for Clinical Laboratory Science (ASCLS) medical scientists are vital in healthcare, uncovering and providing important laboratory analyses that assist physicians in patient diagnosis and treatment. Scientists have a role in monitoring disease progression and treatment monitoring as well. Laboratory scientists utilize technologically advanced biomedical instrumentation and computers to perform laboratory … Continue reading What Is Medical Laboratory Science?