Donath-Landsteiner Antibodies

The history of the DL antibody goes back to the 1900's. It was one of the first recognized forms of immune mediated hemolysis and responsible for inducing Paroxysmal Cold Hemoglobinuria (PCH). PCH is a transient condition, meaning that it comes on when immunoglobulins (Antibodies) are formed in response to a viral, bacterial, or spirochete infection. … Continue reading Donath-Landsteiner Antibodies

DARA-T Workup

Daratumumab (Darzalex) is an IgG1k monoclonal antibody directed against CD38, which is over expressed on the plasma cells in patients with multiple myeloma. Daratumumab binds to CD38 and causes apoptosis through antibody-dependent cellular cytotoxicity or complement-dependent cytotoxicity. In 2015 the FDA approved daratumumab for the treatment of refractory multiple myeloma. Refractory meaning that patients have … Continue reading DARA-T Workup

Blood System Portfolio: Rhesus System

The Rhesus blood system is arguably the second most important blood system behind the ABO system. There are 50 defined blood group antigens, among which the five antigens; D, C, E, c, e are the most significant. Individuals who are Rh positive possess the D antigen and those who are Rh negative lack the D … Continue reading Blood System Portfolio: Rhesus System

Blood Components 101

This will serve as a guide for the specific indications, storage requirements and stability of the different blood components. Storage Whole Blood, Packed Red Blood Cells: 1-6 degrees Celsius. Plasma, Cyroprecipitated AHF: -18 degrees Celsius. Platelets: 20-24 degrees Celsius with continuous gentle agitation. Granulocytes: 20-24 degrees Celsius without agitation. Stability Whole Blood: When refrigerated a … Continue reading Blood Components 101

Blood System Portfolio: ABO Group

The ABO blood group system was first discovered in 1901 by Karl Landsteiner. In 1902 Sturli and Von Decastello discovered the AB group. The ABH antigens do not develop until about 6 weeks of fetal life and the concentration of antigens increases until 3-4 years of age and level out. Antibodies to the ABO blood … Continue reading Blood System Portfolio: ABO Group